Diagnosis of Brachyspira pilosicoli, Brachyspira hyodysenteriae and Brachyspira intermedia in hens and laying hens in the western region of Paraná through bacterial isolation and identification in qPCR
نویسندگان
چکیده
منابع مشابه
Brachyspira intermedia and Brachyspira pilosicoli are commonly found in older laying flocks in Pennsylvania.
Anaerobic intestinal spirochetes (genus Brachyspira) include several species that are recognized as pathogens of poultry. Surveys undertaken in Europe and Australia have shown that layer and breeder flocks are often colonized by the pathogenic species Brachyspira intermedia and Brachyspira pilosicoli, but similar surveys have not been conducted in the United States. In the current study, DNA wa...
متن کاملIntestinal colonization of different Brachyspira spp. in laying hens
BACKGROUND:Avian intestinal spirochetosis (AIS) is causedby spiral-shaped Gram-negative Brachyspira spp. in poultry. It isknown as a cause of diarrhea, low egg production, and increasedoccurrence of dirty eggs in layer hens. OBJECTIVES: In this study,the presence of some Brachyspira spp. was investigated in layinghens. METHODS: A total of 100 cloacal swab samples wereindividually collected from...
متن کاملImmunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces.
Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions. The purpose of the present study was to compare the relative sensitivi...
متن کاملIdentification of genes associated with prophage-like gene transfer agents in the pathogenic intestinal spirochaetes Brachyspira hyodysenteriae, Brachyspira pilosicoli and Brachyspira intermedia. Veterinary Microbiology,
VSH-1 is an unusual prophage-like gene transfer agent (GTA) that has been described in the intestinal spirochaete Brachyspira hyodysenteriae. The GTA does not self-propagate, but it assembles into a virus-like particle and transfers random 7.5 kb fragments of host DNA to other B. hyodysenteriae cells. To date the GTA VSH-1 has only been analysed in B. hyodysenteriae strain B204, in which 11 lat...
متن کاملAntimicrobial susceptibility of porcine Brachyspira hyodysenteriae and Brachyspira pilosicoli isolated in Sweden between 1990 and 2010
BACKGROUND The anaerobic spirochetes Brachyspira hyodysenteriae and Brachyspira pilosicoli cause diarrheal diseases in pigs. Their fastidious nature has hampered standardization of methods for antimicrobial susceptibility testing. For monitoring of antimicrobial susceptibility wild type cutoff values are needed to define where the wild type distribution of MICs ends and no approved cutoffs are ...
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ژورنال
عنوان ژورنال: Pesquisa Veterinária Brasileira
سال: 2019
ISSN: 1678-5150,0100-736X
DOI: 10.1590/1678-5150-pvb-6030